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Transiently and non-invasively magnetized cells are placed in a Cell-Repellent plate. A magnet placed above the plate attracts the cells to the air/medium interface. Magnetic forces bundle the cells together, favouring intercellular interactions at the origin of the extracellular matrix synthesis.
Transiently and non-invasively magnetized cells are placed in a plate and a magnetic holder will be positioned underneath that plate, keeping the cells at the bottom of the well. In a few minutes or even hours, the plate containing the spheroids can be removed from the magnetic holder and cultured over the long term.
Based on the 3D magnetic Spheroid printing, transiently and non-invasively magnetized cells thanks to Nanoshuttle-PL beads are positioned in a ring shape mimicking certain in-vivo patterns and their functions (ex. contraction).
• Nano-particles assembly (50 nm diameter), does not bind any specific receptors, works with all cell types - attaches to the plasma membrane electrostatically
• Consists of biocompatible gold, iron oxide and Poly-L-Lysine
• No effects on proliferation, viability, metablism, oxydative stress, phenotype
• Does not disturb experimental techniques such as immunohistological labelling, fluorescence detection, HR microscopy, Western Blot, PCR/qPCR
Magnetic tool for the individual manipulation of formed spheroids
• Fast, easy and simultaneous transfer of multiple 3D cell cultures without pipetting
• Complete change of media by a simple pick-up-and-drop step
• Simplified co-culture of different cell types
• Ideal for facilitating immunohistochemical staining, blocking and washing of spheroids