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• Catalyses the removal of nucleotides from single-stranded DNA in the 3' -> 5' direction • Does not degrade double-stranded DNA or RNA • Ideal for single-strand primers degradation after PCR prior to DNA sequencing or SNP analysis • Used for single-stranded DNA degradation in a double-stranded DNA preparation • Active in a wide variety of reaction buffers • Available in 4000 and 20000 units formats