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• It is structure-selective enzyme that recoginzes and cleaves mismatched DNA, heteroduplex DNA, cruciform DNA structures, Holliday structures or junctions and more slowly, nicked dsDNA • The cleavage site is the first, second or third phosphodiester bond that is 5' to the mismatch • Ultrapure recombinant enzyme
Possible applications: • Recognition of mismatched DNA in particular in the context of genome editing using the Crispr method • Resolve four-way junction or branched DNA • Detect or cleave heteroduplex and nicked DNA • Available in 250 and 1250 unit format
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