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• Recombinant protein, ultrapure, at an initial concentration of 5 U/?l • Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity • Adds a free adenine to the 3' end of the amplicon (monoadenylation) • Kit with 3 different 10X reaction buffers: without MgCl? (A), with MgCl? (B), with 2 inert dyes for direct deposit (C) • Kit supplied with dNTPs (separate tube)