For security reasons, you will be logged out in 4 minutesCannot be added!Your basket contains a blocked quote and must be finalised before you can order other items.Add to basket...Item added to basket
Express
orderDisplay
my quote
Basket0
Good dealsSolutionsE-catalogs
FacilitatingServicesINFORMATION & CONTACT
0
Error when adding ref. {0} to favouritesError when removing ref. {0} from favourites
BioreactiveReagent for molecular biologyProtein extraction and purification
27-0843-01

27-0843-01 · Prescission™ protease - 2000 units/ml in 50 mM Tris-HCl, 150 mM NaCl, 10mM EDTA, 1 mM DTT and 20 % glycérol)

Sales Unit: 1
Brand: Cytiva
Part Number: 27084301
NACRES Code: NA.56
Know the availability ?Log in
Shipping costs with dry ice - flat rate per shipment
40.00 €
364.00 €
UGAP user? Click here to view the item on ugap.fr
GST fusion protein cleavage enzyme

Used for site-specific separation of the GST tag from proteins:
• expressed using pGEX-T vectors: Thrombin Protease;
• expressed using pGEX-X vectors: Facto Xa Protease;
• expressed using pGEX-6P vectors: PreScission™ Protease.

PreScission Protease: one unit will cleave >90 % of 100 µg of a test GST-fusion protein in 50 mm Tris-HCl, 150 mm NaCl, 1 mm EDTA, 1 mm DTT, pH 7.0 at 5 °C for 16 h.
Thrombin Protease: one unit of enzyme cleaves 90% of 100 ?g of a test GST fusion protein when incubated in 1 × PBS at 22 °C for 16 h
Factor Xa Protease: one unit cleaves >90 % of 100 ?g of a test GST fusion protein when incubated in 1 mm CaCl?, 100 mm NaCl, and 50 mm Tris-HCl (pH 8.0) at 22 °C for 16 h.
Gross weight0,00 kg
Gross dimensions8,00 x 4,00 x 1,00 cm
Customs Code3507909090
UNSPSC Code41106500
en27-0843-01 - User manualen27-0843-01 - Brochurefr27-0843-01 - Safety dataen27-0843-01 - Safety dataGE chromatography guideCan't find the technical documentation you need? Click here to contact our technical hotline

Variant(s)

GSTrap columns are designed for one-step purification of GST-tagged proteins. The columns are prepacked with the matrices:
• Glutathion Sepharose HP: for high-resolution purifications, from a more concentrated sample;
• Glutathion Sepharose FF: for high flow rate purifications;
• Glutathion Sepharose 4B, standard support.

Available in 2 formats: 1 or 5 ml column.

Available in stock : {0}Supply in progressDelivery times on request
17-5130-01 · GSTrap FF, volume: 5 x 1 ml
Sales Unit: 1
Cytiva
Made in Europe
17-5130-02 · GSTrap FF, volume: 2 x 1 ml
Sales Unit: 1
Cytiva
Made in Europe
17-5131-01 · GSTrap FF, volume: 1 x 5 ml
Sales Unit: 1
Cytiva
Made in Europe
17-5131-02 · GSTrap FF, volume: 5 x 5 ml
Sales Unit: 1
Cytiva
Made in Europe
17-5281-01 · GSTrap HP, volume: 5 x 1 ml
Sales Unit: 1
Cytiva
Made in Europe
17-5282-01 · GSTrap HP, volume: 1 x 5 ml
Sales Unit: 1
Cytiva
Made in Europe
17-5282-02 · GSTrap HP, volume: 5 x 5 ml
Sales Unit: 1
Cytiva
Made in Europe
28-4017-45 · GSTrap 4B, volume: 5 x 1 ml
Sales Unit: 1
Cytiva
Made in Europe
28-4017-47 · GSTrap 4B, volume: 1 x 5 ml
Sales Unit: 1
Cytiva
Made in Europe
28-4017-48 · GSTrap 4B, volume: 5 x 5 ml
Sales Unit: 1
Cytiva
Made in Europe
Available in stock : {0}Supply in progressDelivery times on request
28-9365-50 · GSTPrep FF 16/10
Sales Unit: 1
Cytiva
Made in Europe

GST fusion protein cleavage enzyme

Used for site-specific separation of the GST tag from proteins:
• expressed using pGEX-T vectors: Thrombin Protease;
• expressed using pGEX-X vectors: Facto Xa Protease;
• expressed using pGEX-6P vectors: PreScission™ Protease.

PreScission Protease: one unit will cleave >90 % of 100 µg of a test GST-fusion protein in 50 mm Tris-HCl, 150 mm NaCl, 1 mm EDTA, 1 mm DTT, pH 7.0 at 5 °C for 16 h.
Thrombin Protease: one unit of enzyme cleaves 90% of 100 ?g of a test GST fusion protein when incubated in 1 × PBS at 22 °C for 16 h
Factor Xa Protease: one unit cleaves >90 % of 100 ?g of a test GST fusion protein when incubated in 1 mm CaCl?, 100 mm NaCl, and 50 mm Tris-HCl (pH 8.0) at 22 °C for 16 h.

Available in stock : {0}Supply in progressDelivery times on request
27-0846-01 · Thrombin protease, lyophilized powder 500 UI
Sales Unit: 1
Cytiva
Special delivery

You may be also interested in these products

Extraction and purification of RNA, DNA and PCR productsqPCRReagent forProtein extraction and purificationOther enzyme for molecular biologyTransfection reagentReagent for PCRReagent for molecular biologyRestriction endonucleasesAgarose for nucleic acid electrophoresisSequencing reagentBuffer and other reagents for protein electrophoresisPrecast gel for protein electrophoresisReagent for IEFBlotting reagent - antibodyStaining reagent for electrophoresisBuffer for nucleic acid electrophoresisdNTP - deoxyNucleoside TriPhosphatePCR kit and reagentEnzyme for PCRReagent for RT-PCRNucleic acid labelingPrecast gel for nucleic acid electrophoresisDNA size markerAcrylamide for protein electrophoresisNucleic acid quantificationEnzyme and DNA modification kitNucleic acid preparationProteinWater for molecular biologyAmplification reagent for molecular biologyProtein dye for electrophoresisBlotting reagentProtein detection and analysisSurface decontamination - molecular biologyProtein size markerProtein detection and analysis dosageEnzyme for RT-PCRExpression, cloning, and functional analysisReagent for RT-qPCRStability and conservation of RNAPCR grade waterReagent for LAMP-PCREnzyme for qPCRReagent for RT-LAMPNucleic acid electrophoresis and revelationProtein electrophoresisGene editing kitSolvent and chemical for molecular biologyPrimerLysis and protein extraction reagentNucleotideStability and conservation of DNABuffer - amplification