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BioreactiveReagent for molecular biologyProtein extraction and purification
27-0846-01

27-0846-01 · Thrombin protease, lyophilized powder 500 UI

Sales Unit: 1
Brand: Cytiva
Part Number: 27084601
NACRES Code: NA.28
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Shipping costs with dry ice - flat rate per shipment
40.00 €
164.00 €
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GST fusion protein cleavage enzyme

Used for site-specific separation of the GST tag from proteins:
• expressed using pGEX-T vectors: Thrombin Protease;
• expressed using pGEX-X vectors: Facto Xa Protease;
• expressed using pGEX-6P vectors: PreScission™ Protease.

PreScission Protease: one unit will cleave >90 % of 100 µg of a test GST-fusion protein in 50 mm Tris-HCl, 150 mm NaCl, 1 mm EDTA, 1 mm DTT, pH 7.0 at 5 °C for 16 h.
Thrombin Protease: one unit of enzyme cleaves 90% of 100 ?g of a test GST fusion protein when incubated in 1 × PBS at 22 °C for 16 h
Factor Xa Protease: one unit cleaves >90 % of 100 ?g of a test GST fusion protein when incubated in 1 mm CaCl?, 100 mm NaCl, and 50 mm Tris-HCl (pH 8.0) at 22 °C for 16 h.
Gross weight0,03 kg
Gross dimensions3,20 x 3,20 x 7,60 cm
Customs Code3002120000
UNSPSC Code41106500
fr27-0846-01 - Manufacturer data sheetfr27-0846-01 - Safety dataen27-0846-01 - Safety dataGE chromatography guideCan't find the technical documentation you need? Click here to contact our technical hotline

Variant(s)

GSTrap columns are designed for one-step purification of GST-tagged proteins. The columns are prepacked with the matrices:
• Glutathion Sepharose HP: for high-resolution purifications, from a more concentrated sample;
• Glutathion Sepharose FF: for high flow rate purifications;
• Glutathion Sepharose 4B, standard support.

Available in 2 formats: 1 or 5 ml column.

Available in stock : {0}Supply in progressDelivery times on request
17-5130-01 · GSTrap FF, volume: 5 x 1 ml
Sales Unit: 1
Cytiva
Made in Europe
17-5130-02 · GSTrap FF, volume: 2 x 1 ml
Sales Unit: 1
Cytiva
Made in Europe
17-5131-01 · GSTrap FF, volume: 1 x 5 ml
Sales Unit: 1
Cytiva
Made in Europe
17-5131-02 · GSTrap FF, volume: 5 x 5 ml
Sales Unit: 1
Cytiva
Made in Europe
17-5281-01 · GSTrap HP, volume: 5 x 1 ml
Sales Unit: 1
Cytiva
Made in Europe
17-5282-01 · GSTrap HP, volume: 1 x 5 ml
Sales Unit: 1
Cytiva
Made in Europe
17-5282-02 · GSTrap HP, volume: 5 x 5 ml
Sales Unit: 1
Cytiva
Made in Europe
28-4017-45 · GSTrap 4B, volume: 5 x 1 ml
Sales Unit: 1
Cytiva
Made in Europe
28-4017-47 · GSTrap 4B, volume: 1 x 5 ml
Sales Unit: 1
Cytiva
Made in Europe
28-4017-48 · GSTrap 4B, volume: 5 x 5 ml
Sales Unit: 1
Cytiva
Made in Europe
Available in stock : {0}Supply in progressDelivery times on request
28-9365-50 · GSTPrep FF 16/10
Sales Unit: 1
Cytiva
Made in Europe

GST fusion protein cleavage enzyme

Used for site-specific separation of the GST tag from proteins:
• expressed using pGEX-T vectors: Thrombin Protease;
• expressed using pGEX-X vectors: Facto Xa Protease;
• expressed using pGEX-6P vectors: PreScission™ Protease.

PreScission Protease: one unit will cleave >90 % of 100 µg of a test GST-fusion protein in 50 mm Tris-HCl, 150 mm NaCl, 1 mm EDTA, 1 mm DTT, pH 7.0 at 5 °C for 16 h.
Thrombin Protease: one unit of enzyme cleaves 90% of 100 ?g of a test GST fusion protein when incubated in 1 × PBS at 22 °C for 16 h
Factor Xa Protease: one unit cleaves >90 % of 100 ?g of a test GST fusion protein when incubated in 1 mm CaCl?, 100 mm NaCl, and 50 mm Tris-HCl (pH 8.0) at 22 °C for 16 h.

Available in stock : {0}Supply in progressDelivery times on request
27-0843-01 · Prescission™ protease - 2000 units/ml in 50 mM Tris-HCl, 150 mM NaCl, 10mM EDTA, 1 mM DTT and 20 % glycérol)
Sales Unit: 1
Cytiva
Special delivery

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